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pet28a btkv cas9  (Integrated DNA Technologies)


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    Structured Review

    Integrated DNA Technologies pet28a btkv cas9
    CRISPR editing efficiency in Bemisia tabaci
    Pet28a Btkv Cas9, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 99/100, based on 4157 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet28a+btkv+cas9/Cas9+Nuclease/pmc07194319-55-28-37
    Average 99 stars, based on 4157 article reviews
    pet28a btkv cas9 - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "CRISPR-Cas9-Based Genome Editing in the Silverleaf Whitefly ( Bemisia tabaci )"

    Article Title: CRISPR-Cas9-Based Genome Editing in the Silverleaf Whitefly ( Bemisia tabaci )

    Journal: The CRISPR Journal

    doi: 10.1089/crispr.2019.0067

    CRISPR editing efficiency in Bemisia tabaci
    Figure Legend Snippet: CRISPR editing efficiency in Bemisia tabaci

    Techniques Used: CRISPR, Injection, Sampling

    Targeting of molecular cargo to Bemisia tabaci ovaries. The BtKV peptide targets molecular cargo (mCherry-Cas9 fusion protein) into B. tabaci ovaries and is visible in dissected mature oocytes 24 h post injection (top). No signal is visible in control ovaries (bottom).
    Figure Legend Snippet: Targeting of molecular cargo to Bemisia tabaci ovaries. The BtKV peptide targets molecular cargo (mCherry-Cas9 fusion protein) into B. tabaci ovaries and is visible in dissected mature oocytes 24 h post injection (top). No signal is visible in control ovaries (bottom).

    Techniques Used: Injection

    Related Articles

    Residue:

    Article Title: CRISPR/Cas9-based genome editing in the silverleaf whitefly ( Bemisia tabaci )
    Article Snippet: .. We identified a lysine conserved across B. tabaci sequences and a fully conserved valine residue that defined the ends of the targeting sequence (“BtKV”) ( ). pET28A-BtKV-Cas9 was constructed from pET28-P2C-Cas9 ( ) and a gblock (IDT) of BtKV-mCherry. pET28A-P2C-Cas9 was digested with restriction enzymes BamHI and SalI. .. A gblock of BtKV fused to mCherry was synthesized and inserted into the digested pET28-P2C-Cas9 using In-Fusion Cloning (Takara).

    Article Title: CRISPR-Cas9-Based Genome Editing in the Silverleaf Whitefly ( Bemisia tabaci )
    Article Snippet: .. We identified a lysine conserved across B. tabaci sequences and a fully conserved valine residue that defined the ends of the targeting sequence (“BtKV”; Supplementary Fig. S1 ). pET28A-BtKV-Cas9 was constructed from pET28-P2C-Cas9 10 and a gblock (IDT) of BtKV-mCherry. pET28A-P2C-Cas9 was digested with restriction enzymes BamHI and SalI. .. A gblock of BtKV fused to mCherry was synthesized and inserted into the digested pET28-P2C-Cas9 using In-Fusion Cloning (Takara Bio).

    Sequencing:

    Article Title: CRISPR/Cas9-based genome editing in the silverleaf whitefly ( Bemisia tabaci )
    Article Snippet: .. We identified a lysine conserved across B. tabaci sequences and a fully conserved valine residue that defined the ends of the targeting sequence (“BtKV”) ( ). pET28A-BtKV-Cas9 was constructed from pET28-P2C-Cas9 ( ) and a gblock (IDT) of BtKV-mCherry. pET28A-P2C-Cas9 was digested with restriction enzymes BamHI and SalI. .. A gblock of BtKV fused to mCherry was synthesized and inserted into the digested pET28-P2C-Cas9 using In-Fusion Cloning (Takara).

    Article Title: CRISPR-Cas9-Based Genome Editing in the Silverleaf Whitefly ( Bemisia tabaci )
    Article Snippet: .. We identified a lysine conserved across B. tabaci sequences and a fully conserved valine residue that defined the ends of the targeting sequence (“BtKV”; Supplementary Fig. S1 ). pET28A-BtKV-Cas9 was constructed from pET28-P2C-Cas9 10 and a gblock (IDT) of BtKV-mCherry. pET28A-P2C-Cas9 was digested with restriction enzymes BamHI and SalI. .. A gblock of BtKV fused to mCherry was synthesized and inserted into the digested pET28-P2C-Cas9 using In-Fusion Cloning (Takara Bio).

    Construct:

    Article Title: CRISPR/Cas9-based genome editing in the silverleaf whitefly ( Bemisia tabaci )
    Article Snippet: .. We identified a lysine conserved across B. tabaci sequences and a fully conserved valine residue that defined the ends of the targeting sequence (“BtKV”) ( ). pET28A-BtKV-Cas9 was constructed from pET28-P2C-Cas9 ( ) and a gblock (IDT) of BtKV-mCherry. pET28A-P2C-Cas9 was digested with restriction enzymes BamHI and SalI. .. A gblock of BtKV fused to mCherry was synthesized and inserted into the digested pET28-P2C-Cas9 using In-Fusion Cloning (Takara).

    Article Title: CRISPR-Cas9-Based Genome Editing in the Silverleaf Whitefly ( Bemisia tabaci )
    Article Snippet: .. We identified a lysine conserved across B. tabaci sequences and a fully conserved valine residue that defined the ends of the targeting sequence (“BtKV”; Supplementary Fig. S1 ). pET28A-BtKV-Cas9 was constructed from pET28-P2C-Cas9 10 and a gblock (IDT) of BtKV-mCherry. pET28A-P2C-Cas9 was digested with restriction enzymes BamHI and SalI. .. A gblock of BtKV fused to mCherry was synthesized and inserted into the digested pET28-P2C-Cas9 using In-Fusion Cloning (Takara Bio).



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    Integrated DNA Technologies pet28a btkv cas9
    CRISPR editing efficiency in Bemisia tabaci
    Pet28a Btkv Cas9, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    CRISPR editing efficiency in Bemisia tabaci

    Journal: The CRISPR Journal

    Article Title: CRISPR-Cas9-Based Genome Editing in the Silverleaf Whitefly ( Bemisia tabaci )

    doi: 10.1089/crispr.2019.0067

    Figure Lengend Snippet: CRISPR editing efficiency in Bemisia tabaci

    Article Snippet: We identified a lysine conserved across B. tabaci sequences and a fully conserved valine residue that defined the ends of the targeting sequence (“BtKV”; Supplementary Fig. S1 ). pET28A-BtKV-Cas9 was constructed from pET28-P2C-Cas9 10 and a gblock (IDT) of BtKV-mCherry. pET28A-P2C-Cas9 was digested with restriction enzymes BamHI and SalI.

    Techniques: CRISPR, Injection, Sampling

    Targeting of molecular cargo to Bemisia tabaci ovaries. The BtKV peptide targets molecular cargo (mCherry-Cas9 fusion protein) into B. tabaci ovaries and is visible in dissected mature oocytes 24 h post injection (top). No signal is visible in control ovaries (bottom).

    Journal: The CRISPR Journal

    Article Title: CRISPR-Cas9-Based Genome Editing in the Silverleaf Whitefly ( Bemisia tabaci )

    doi: 10.1089/crispr.2019.0067

    Figure Lengend Snippet: Targeting of molecular cargo to Bemisia tabaci ovaries. The BtKV peptide targets molecular cargo (mCherry-Cas9 fusion protein) into B. tabaci ovaries and is visible in dissected mature oocytes 24 h post injection (top). No signal is visible in control ovaries (bottom).

    Article Snippet: We identified a lysine conserved across B. tabaci sequences and a fully conserved valine residue that defined the ends of the targeting sequence (“BtKV”; Supplementary Fig. S1 ). pET28A-BtKV-Cas9 was constructed from pET28-P2C-Cas9 10 and a gblock (IDT) of BtKV-mCherry. pET28A-P2C-Cas9 was digested with restriction enzymes BamHI and SalI.

    Techniques: Injection